System and method for sub-surface fluorescence imaging

ABSTRACT

Methods for quantifying fluorescence and optical properties in a turbid medium such as tissue. Devices and systems suitable for the methods are also disclosed.

CROSS-REFERENCE TO RELATED APPLICATIONS

The present disclosure claims priority from U.S. Provisional PatentApplication No. 61/297,969, filed Jan. 25, 2010, the entirety of whichis hereby incorporated by reference.

TECHNICAL FIELD

The present disclosure relates to devices for quantifying fluorescenceand optical properties. In particular, the present disclosure relates toprobe devices and methods for quantifying fluorescence in an opticallyturbid medium, such as tissue, using optical properties measurements.

BACKGROUND

The present disclosure is related to fluorescence imaging andspectroscopy, in particular the use of fluorescence to detect and/ortreat cancer by marking tumor cells with an appropriate fluorescingagent. One difficulty in using fluorescence for medical diagnostics andtherapeutics is quantifying the fluorescence in tissue. Fluorescencesignals are strongly affected by variations in the tissue absorption andtransport scattering properties (i.e. tissue optical properties),whereas often the objective is to quantify the fluorescence based onfluorophore concentration alone.

Fluorescence measurement is of interest for applications such asphotosensitizer dosimetry during photodynamic therapy (Finlay et al.2006), fluorescence image-guided surgery (Stummer et al. 1998),detection of cancerous or dysplastic lesions (Muller et al., 2001) andin time kinetics studies of fluorescing drugs (Sroka et al. 1996). Theshape and intensity of the fluorescence spectrum contain usefulinformation on the identity and abundance of fluorophores in tissue.However, accuracy of quantitative fluorescence measurement iscomplicated by the distorting effects of light absorption and scatteringby the tissue and the variations in measurement geometry (e.g.detector-to-tissue surface distance). Untangling these confoundingeffects is important for quantitative analysis of fluorescence.

Some methods have been developed in an attempt to diminish thesedistorting effects to better utilize fluorescence information. Many ofthese techniques use a diffuse reflectance signal to correct thefluorescence signal from optical properties variation. Wu and coworkershave developed a fluorescence photon migration model to produce arelation with the diffuse reflectance that can be exploited to extractthe quantitative fluorescence in tissue (Wu et al. 1993). On a differenttack, a single optical fiber may be used for both source and collection,the concept being that detectable fluorescence events occur so close tothe fiberoptic tip that absorption and scattering effects are minimal,analogous to how these effects are minimal for very thin tissue sections(Diamond et al. 2003).

Empirical methods with similar themes have also been developed. Thesingle fiber method was used in conjunction with an empirically-derivedcorrection factor dependent on the optical properties at the emissionwavelength to further compensate for high tissue attenuation in theprostate during PDT studies (Finlay et al. 2006). Afluorescence/reflectance ratio has been used to quantify fluorophoreconcentration, but with the fluorescence and reflectance measured atdifferent source-collector distances (Weersink et al. 2001). In all ofthe above methods, the excitation source operates in the region of lowtissue absorption, which invalidates their use in the UV-blue-green endof the spectrum (i.e. approximately from 350-575 nm), where a very largesubset of fluorophores have fluorescence absorption maxima, such asporphyrins, background autofluorescence and a multitude of artificialfluorescent dyes.

Ex vivo extraction techniques have also been developed that are based onhomogenizing the tissue and diluting the analyte to the point thateffects due to optical scattering and absorption are negligible (Lilgeet al. 1997). These procedures are relatively time-consuming and open toerror due to tissue handling or cryofreezing for post-processing. Itwould be useful to provide an in situ fluorometric approach that hasapplicability to a wide variety of fluorophores and tissues.

SUMMARY

The present disclosure describes a device for quantifying fluorescenceand optical properties in a turbid medium, such as tissue. Inparticular, the device may be in the form of a fiberoptic probe for usein fluorometric applications in tissue. The present disclosure alsodescribes an associated system for controlling optical signals to andfrom the probe and algorithms to quantify optical parameters from theprobe measurements. The disclosed device may be used as an in vivoinstrument to quantify fluorescence in tissue. The device and associatedsystems and methods may compensate for the effects of tissue opticalproperty variation on the perceived fluorescence.

In some examples, the fiberoptic probe generally has a handle attachedto a hypodermic needle-like probe that contacts tissue, which may beflat and includes a linear array of fiberoptics at the tip.Alternatively, the tip may be configured to have an angle or taper suchthat it may more easily push through tissue, for example forinterstitial measurement, similar to a hypodermic needlepoint. A seriesof excitation light sources are routed through the probe tip via asource fiberoptic and the resulting reflectance and/or fluorescencespectra are measured at a distance through a detector fiber. In thisway, measurements of the tissue fluorescence and white light reflectance(reflectance may be taken at varying source-collector fiberopticdistances) can be made. Applying an appropriate model of lightinteraction with tissue, the quantitative fluorescence, absorption andtransport scattering properties (μ_(a) and μ_(s)′) of the tissue can bedetermined, as well as other physiological metrics such as tissueoxygenation and hemoglobin concentration.

In some examples, the device includes: a handheld probe culminating in anarrow tip that contacts the tissue; fiberoptics bundled into the probetip and probe handle and leading to a control system; the fiberoptics atthe tip arranged in a linear array. The device may be used with: acontrol system that routes optical signals to and from the probe handle;a computer that is connected to the control system. The device may beused with algorithms to compute the optical properties and derive thequantitative fluorescence. The system may provide the software on thecomputer to handle the algorithm computation, data acquisition andcontrol; and a user interface to control the settings of the dataacquisition.

The disclosed device, system and method may be used to extract thequantitative fluorescence spectrum (i.e. the fluorescence spectrumcorrected for optical properties effects), and consequently fluorophoreconcentration, using a fiberoptic probe that comes into contact with oris buried interstitially within the tissue. The optical properties,diffuse reflectance spectrum and measured fluorescence spectrum (i.e.the raw, uncorrected fluorescence measurement that is distorted byoptical properties effects) are inputs to the model. Since the tissueoptical properties need to be known, a method to extract these is alsodescribed, that may be referred to as spectrally-constrained diffusereflectance.

In some aspects, there is provided an optical probe for quantifyingfluorescence and optical properties in tissue, the probe comprising: aprobe body and a probe tip at a distal end of the probe body, the probetip being configured to substantially contact a target surface; at leastone detector at the probe tip for detecting fluorescence emission orreflectance wavelengths from the target surface; at least onefluorescence excitation source at the probe tip for providingfluorescence excitation light to the target surface, each one of the atleast one fluorescence excitation source being at a known distance fromeach one of the at least one detector; at least two broadband sources atthe probe tip for providing broadband wavelengths to the target surface,each one of the at least two broadband sources being at a known distancefrom each one of the at least one detector; wherein the device isconfigured to communicate signals from the at least one detectorrepresenting the detected wavelengths to a processing device.

In some aspects, there is provided a method for quantifying opticalproperties in a turbid medium, the method comprising: providingfluorescence emission and reflectance wavelengths detected from a targetsurface, each of the detected wavelengths being associated with arespective known distance between a respective excitation source givingrise to the respective detected wavelength and a detector detecting therespective detected wavelength, the known distances being predeterminedto enable calculation of a desired range of values for the opticalproperties; and calculating the optical properties based on the detectedwavelengths and the respective known distances, and based on a model oflight interaction with the turbid medium, the model limiting a range ofcalculated values for the optical properties.

In some aspects, there is provided a method for quantifying fluorescencein a turbid medium, the method comprising: providing fluorescenceemission and reflectance wavelengths detected from a target surface,each of the detected wavelengths being associated with a respectiveknown distance between a respective excitation source giving rise to therespective detected wavelength and a detector detecting the respectivedetected wavelength, the known distances being predetermined tocorrespond to a desired range of values for the optical properties;providing quantified optical properties corresponding to the detectedwavelengths; and calculating the fluorescence based on the detectedwavelengths and the corresponding optical properties, based on knownrelationships between the detected fluorescence emission wavelengths andthe detected reflectance wavelengths arising from the known distances,and based on a model of light interaction with the turbid medium, themodel limiting a range of calculated values for the optical properties.In some examples, the method for quantifying fluorescence may alsoinclude the method for quantifying optical properties described above.

In some aspects, there is provided a system for quantifying opticalproperties in tissue, the system comprising: the probe described above;a plurality of light sources for providing fluorescence excitation andbroadband wavelengths to the fluorescence excitation source andbroadband sources; a spectrometer for measuring wavelengths detected bythe detector; and a controller for controlling fluorescence emission anddetection by the sources and the detector; wherein the system is incommunication with a processing device configured for calculating atleast one optical property based on the measured wavelengths.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 is a schematic of an example device for quantifying fluorescenceand optical properties;

FIG. 2 is a schematic of another example device for quantifyingfluorescence and optical properties;

FIG. 3 is an end-on view of an example probe tip suitable for theexample device of FIG. 1 or FIG. 2;

FIG. 4 is an end-on view of another example probe tip suitable for theexample device of FIG. 1 or FIG. 2;

FIG. 5 is a schematic of an example system suitable for use with theexample device of FIG. 1 or FIG. 2;

FIG. 6 a and FIG. 6 b show photographs of an example device and anexample system for quantifying fluorescence and optical properties;

FIG. 7 shows a schematic of example fiberoptic-mediated fluorescencedetection;

FIG. 8 shows a schematic of another example fiberoptic-mediatedreflectance detection;

FIG. 9 is a diagram illustrating an example method for quantifyingfluorescence and optical properties;

FIG. 10 shows a graph of reflectance versus μ_(s)′ for an examplefiberoptic distance of 500 μm;

FIG. 11 shows a bar graph for three example fiberoptic distances (r=260,520 and 780 μm) and their range of validity according to an examplemodel for quantifying optical properties;

FIG. 12 is a table showing phantom optical properties for an examplestudy of an example system and method for quantifying opticalproperties;

FIG. 13 shown example phantom fluorescence spectra (a) uncorrected foroptical properties variation and (b) corrected for the effects of theoptical properties;

FIG. 14 shows a) measured fluorescence and b) estimated fluorophoreconcentration versus true PpIX concentration, from an example study;

FIG. 15 shows images of example PpIX phantoms A-I;

FIGS. 16A and 16B displays fluorescence measurements from an example exvivo mouse experiment;

FIG. 17 is a table showing baseline characteristics for patients in anexample study of an example system and method for quantifying opticalproperties;

FIG. 18 shows plots of example optical properties spectra andreflectance data from an example study of an example system and methodfor quantifying optical properties;

FIG. 19 shows plots of example fluorescence data from a patient with ameningioma, measured using an example device for quantifyingfluorescence and optical properties;

FIG. 20 shows example fluorescence images and corresponding examplefluorescence spectra from a patient with a glioma, measured using anexample device for quantifying fluorescence and optical properties;

FIG. 21 is a table showing comparisons of example PpIX concentrationlevels from an example study of an example system and method forquantifying optical properties;

FIG. 22 is a table showing example diagnostic variables tested in anexample study of an example system and method for quantifying opticalproperties;

FIG. 23 shows plots of example in vivo quantitative fluorescence from anexample study of an example system and method for quantifying opticalproperties;

FIG. 24 shows plots of example diagnostic variables tested in an examplestudy of an example system and method for quantifying opticalproperties; and

FIGS. 25-29 are tables of example data for diagnostic variables testedin an example study of an example system and method for quantifyingoptical properties.

DETAILED DESCRIPTION

The present disclosure describes a device, system and method that may beused for recovering the quantitative fluorescence, individualfluorophore concentrations, and/or other optically-determinedphysiological metrics (e.g., in the case of tissue, the tissueoxygenation, hemoglobin concentration, etc. may be recovered). Anymeasureable tissue fluorescence may be significantly affected by themeasurement geometry and tissue optical properties. For example,fluorescence image intensity (in epi-illumination mode) varies withcamera-to-tissue distance approximately according to an inverse law. Anincreased blood volume significantly attenuates fluorescence intensitydue to the high absorption of hemoglobin. Attempts to quantify thefluorescence without taking into account these factors may lead toincorrect interpretation.

The disclosed device, system and method may help reduce, minimize oreliminate the issue of varying measurement geometry by fixing the sourceand detector geometry (e.g., as mediated with fiberoptics embedded in acylindrical probe head) and by making contact with the tissue duringmeasurement. As well, measurement of optical properties in combinationwith a novel fluorescence model and algorithm may be used to remove thedistorting effects of the tissue optical properties.

The present disclosure describes a combination of the “hardware”equipment (e.g., fiberoptic probe, control system and computinghardware) required to produce the spectroscopic measurements and the“software” algorithms to process the raw data to reconstruct thequantitative fluorescence spectrum, fluorophore concentration(s),optical properties and/or physiological metrics such as tissueoxygenation and hemoglobin concentration in the case of tissue. Thesoftware part may be further divided into the optical propertiescalculation part and the quantitative fluorescence calculation part. Theresult from the optical properties calculation is used in thequantitative fluorescence part; however, any suitable method ofobtaining optical properties may be used in the quantitativefluorescence part. Thus, although the two are discussed together, thequantitative fluorescence calculation may be performed independent ofthe described optical properties calculation, even though the result ofthe optical properties calculation is used in the quantitativefluorescence calculation. The following description discusses thehardware and software parts separately; however, both parts may be usedtogether.

The following is a description of an example of the hardware portion.FIG. 1 illustrates an example of the device, in this case a portablefiberoptic probe. In this example, the handle 9 is connected to theprobe barrel 1 on one end and the fiberoptic lead 10 leading back to thecontrol system (not shown) where the optical signals are received. Thetip of probe barrel 1 is the part that actually contacts the tissue siteof interest. Examples of suitable tip geometries are shown in FIG. 3 andFIG. 4. The fiberoptic lead 10 includes, in this example configuration,four separate optical fibers, which are split apart from the main leadinto four separate leads by part 11. The four separate leads terminateinto fiberoptic connectors 12, 13, 14 and 15. The fiberoptic lead 10connects the probe via connectors 12,13,14 and 15 to the control system(see FIG. 5).

The probe may also be catheterizable, as shown in the example device ofFIG. 2. The example shown in FIG. 2 is similar to that of FIG. 1,however in FIG. 2, the probe barrel 1 is connected directly to thefiberoptic lead 10 to enable catheterization. Other such modificationsmay be made to the device to accommodate its use in various procedures.

In some examples, the probe tip geometry can take the form shown in FIG.3. FIG. 3 is an end-on view of an example of the probe tip. The fibersare aligned in a linear array with varying distances from each other. Inthis example configuration, adjacent fibers are 260 μm apart. Probebarrel 1 has the optical fibers built into it, for example held in placewith epoxy 8 or any other suitable fixative, such that their geometrywith respect to each other is well-defined and relatively unchangeable.Fibers 2-5 in this case are connected to fiberoptic connectors 12-15(see FIG. 1). The probe tip may be ground flat and polished in order tomaximize signal from the probe tip.

The probe tip may have more than four fibers, such as that as shown inthe example tip of FIG. 4. In this example, six fibers are shown,although more or less fibers may be used. The fibers are located atknown distances from each other. One source-collector pair is used tomeasure the tissue fluorescence spectrum (for example, in the exampleprobe tip of FIG. 4, this may be fiber 2′ as the fluorescence excitationsource and fiber 3′ as the detector). The other fibers are used tomeasure the diffuse reflectance spectrum at different fiberopticseparation distances (for example, in FIG. 4, the detector fiber 3′measures the diffuse reflectance spectrum at different distances aswhite light is sequentially sent through fibers 4′, 5′, 6′ and 7′).Although in the examples shown, the source and detection fibers arearranged in a linear manner, other fiber configurations may be possible.For example, the fibers may be arranged in a circular, staggered orrandom configuration, as long as their relative distances r are defined.In the example method and model described below, it may be useful tohave at least one white light source fiber at the same distance from thedetector fiber as at least one fluorescence excitation source fiber.There may be more than one fluorescence excitation source fiberprovided, with possibly different fluorescence excitation wavelengthsfor different fluorescence excitation source fibers. In some examples,it may be useful to have all fluorescence excitation source fibers thesame distance away from the detector. The use of different excitationwavelengths may allow for excitation of a variety of fluorophores. Forexample, one way to implement multiple fluorescence excitation sourcefibers into the example shown in FIG. 3 may be to add fluorescenceexcitation source fibers 260 μm above and beneath the detector fiber 3.

The use of multiple fiberoptic distances for measuring the diffusereflectance is related to techniques in measuring the tissue opticalproperties (recall that the tissue optical properties need to beestimated to feed into the quantitative fluorescence algorithm).Spectrally-constrained diffuse reflectance methods have been developedthat allow the use of a single fiberoptic source-collector pair (forexample, see FIG. 8). The source fiber delivers broadband or white lightand the diffuse reflectance spectrum is detected by the collector fiberlocated at a distance, r. Since there is only one reflectancemeasurement per wavelength, λ, solving for μ_(a) and μ_(s)′ relies uponspectral constraints, i.e. applying a priori knowledge of the shapes ofμ_(a)(λ) and μ_(s)′(λ) in a forward model, which can then be used tosolve for the absolute coefficient values. A challenge with thespectrally-constrained diffuse reflectance method is the relativelylimited dynamic range of μ_(a) and μ_(s)′ that can be measured with asingle source-collector distance. Therefore, one of the purposes ofusing multiple source-collector distances for measuring the diffusereflectance is to span a large dynamic range of optical properties.Since each distance spans a unique range over which μ_(a) and μ_(s)′ canbe measured, overlap of the reflectance measurements at multipledistances may help to extend the dynamic range beyond that of eachdistance separately.

The way that reflectance behaves with increasing reduced scatteringcoefficient, μ_(s)′, is to increase with increasing μ_(s)′, peak, andthen decrease. An example of this is shown in FIG. 10, which is anexample graph of reflectance versus μ_(s)′ for a fiberoptic distance of500 μm. The peak is indicated as a cut-off point for the “usefulness” ofthe reflectance for estimating the optical properties, since only thepart which is monotonically increasing or the part that is monotonicallydecreasing can be used, but not both. It has been found that themonotonically increasing part is more sensitive to changes in μ_(s)′, sothis part is used for optical properties measurement in the examplesdescribed. However, it should be understood that the monotonicallydecreasing part may also be used. The reflectance peak of FIG. 10 is atrelatively high μ_(s)′ for small fiberoptic distance, r; hence, closefiberoptic distances (in this example, r<2 mm) are used to span a largedynamic range of μ_(s)′.

In the disclosed device, there are multiple optical fibers for measuringthe diffuse reflectance in order to span a large range of tissue opticalproperties. Since a diffusion theory model is used for opticalproperties extraction (described below), there is a lower bound ofvalidity of μ_(s)′ for a given r such that diffusion theory is valid. Aswell, if the monotonically increasing part (with respect to μ_(s)′) isbeing used then the reflectance peak (see FIG. 10) represents an upperbound for a given r. Therefore, different r distances have differentdynamic ranges for optical properties measurement. For example, in thecase of r=260, 520 and 780 μm, these dynamic ranges are shown in FIG.11. FIG. 11 shows a bar graph for three fiberoptic distances (r=260, 520and 780 μm) and their range of validity according to the reducedscattering coefficient, μ_(s)′. The lower bound is due to the diffusiontheory model breaking down at low μ_(s)′; the upper bound is due to thereflectance peak. This figure shows the need for multiple fiberopticdistances, r, to span a large overall dynamic range of opticalproperties. The technique of spectrally constrained diffuse reflectanceusing multiple r to expand the dynamic range of optical propertiesmeasurement may differ from conventional methods.

An alternative purpose to having multiple source-collector distances formeasuring tissue optical properties is to use a technique calledspatially-resolved diffuse reflectance. Essentially, the reflectancemeasurements at multiple r can constrain the solution such that μ_(a)and μ_(s)′ can be solved for in a non-linear least squares solution.This solution may have some drawbacks, including, for example:relatively slower acquisition times and larger r values, which maynecessitate bulkier probe head diameters; and a less robust solutionthat may lead to spurious results in a dynamically changing biologicalenvironment (e.g. breathing and pulsatile blood flow).Spatially-resolved diffuse reflectance is, however, still a viabletechnique for extracting tissue optical properties for the purpose ofinputting into the quantitative fluorescence algorithm.

In operation, the probe sequentially sends fluorescence excitation lightand broadband light (for each r distance) into the tissue to obtain thefluorescence and diffuse reflectance spectra, respectively. Thefluorescence spectrum depends on five main parameters, the absorptionand transport scattering coefficients at the excitation wavelength,μ_(a,x) and μ_(s,x)′, and the emission wavelength, μ_(a,m) and μ_(s,m)′,and fluorophore content. In this disclosure, the x and m suffices areused to denote excitation and emission, respectively. The reflectancespectrum depends on the wavelength-dependent absorption and scatteringcoefficients, μ_(a)(λ) and μ_(s)′(λ). Based on a diffusion theory modelof light transport in tissue, all of these quantities can be calculatedfrom the fluorescence and reflectance measurements. As well asfluorescence quantification being achieved, many other useful parameterscan be calculated from the data, such as tissue oxygenation, hemoglobinconcentration and a metric of the abundance of optical scatterers intissue such as cells, organelles and the extracellular matrix.

FIG. 5 shows a schematic of an example system suitable for use with thedevice described above. This example control system may be used tocontrol optical signals flowing into and out of the example probe. Adata output card 24 communicates with and is controlled by a processingdevice, such as an external computer (not shown). The system alsoincludes a spectrometer 19 that communicates with the processing device.The data output card 24 controls multiple light-emitting-diodes (LEDs)16, 17 and 18 that provide the excitation light for the fluorescence andwhite light reflectance spectral measurements. The data output card 24communicates to a processing device (e.g., an example control computer)for data acquisition and control via a data output port 22. Fiberopticconnector ports 25, 26, 27 and 28 are used to connect the fiberopticprobe to the control system. The detector fiber (for example, in FIG. 3and FIG. 4 the fiber in the probe tip is 3 and 3′, respectively) isplugged into the spectrometer 19. Alternatively, a detector with anappropriate emission filter may be used for single-wavelength detection.Another possibility for detection would be a charge-coupled device (CCD)in combination with a diffraction grating or prism. In all detectionconfigurations, there may be the option to insert a longpass filter infront of the detector to reject the fluorescence excitation. Thefluorescence excitation fiber (for example, in FIG. 3 and FIG. 4 this isfiber 2 and 2′) is connected to a narrow wavelength band light source,such as an LED 16 as shown (in some examples, a laser or filtered whitelight source may also be used), through port 25. Additional lightsources 17, 18 (for example, LEDs, halogen lamps or other lamps) for thediffuse reflectance measurements send light sequentially through ports26 and 27. A power supply 20 supplies appropriate voltage and current toall devices (e.g., LEDs and any other electronics) as necessary. Anenclosure 23 contains all electrical devices in such a manner thatexternal devices (e.g., the fiberoptic probe and the computer) may beconnected to it easily, and that the enclosure is made electricallysafe. The enclosure 23 may provide a port for the external electricalsupply 21 and a data port 22 (e.g., to connect to a computer).

Although the example system has been described with certain components,variations may be possible. The system may have more LEDs than thoseshown. The system may be portable (e.g., the system may include aportable power source such as a battery, and may include an embeddedmicroprocessor rather than communicating with an external processingdevice). Rather than a data output card in communication with anexternal computer, the system may include a processor for performing thefunctions of these components, for example as described above. In someembodiments, the system may communicate wirelessly with an externalprocessing device rather than through data ports. The system may beadapted or configured to carry out a method for quantifying opticalproperties, for example by carrying out calculations based on the modeldescribed below. In other examples, the system may communicate with anexternal processing device to carry out such calculations.

Example Device and System

An example of the above-described device and system is described below.The example is based on the examples shown in FIG. 1, FIG. 3 and FIG. 5.The example may be suitable for pre-clinical and/or clinical evaluationof quantitative fluorescence, for example for delineation of braintumors during resection surgery. A photograph of the example fiberopticprobe is shown next to a 28 mm diameter Canadian two-dollar coin (forscale) in FIG. 6 a. In the example shown, the probe includes afiberoptic lead, in this case a 3 m long fiberoptic lead. In thisexample, a linear array of four optical fibers (ThorLabs, Newton, N.J.,USA), spaced apart every 260 μm, were epoxied into an 18 Ga hypodermicneedle tube. The silica core of the fiber was 200 μm, with a numericalaperture of 0.22. The hypodermic needle part was affixed to a stainlesssteel handle, with the four fibers extending 3 m to SMA 905 fiberopticconnectors.

The example system is shown in FIG. 6 b. In this example, the systemincludes the probe, and a control system, in communication with anexternal processing device, in this case a data acquisition computer.The system may also include an isolation transformer, as shown, foradditional electrical isolation, which may be useful when the system isto be used in the operating theatre. The control system directs the flowof optical signals into and out of the probe handle. The white lightsources for the diffuse reflectance measurements and the source forfluorescence excitation (in this example, at around 405 nm) in thisexample are LEDs (LEDengin, Santa Clara, Calif., USA), controlled bycomputer via a data output card (Measurement Computing, Norton, Mass.,USA). The fluorescence LED was filtered with a 550 nm shortpass filter(Edmund Optics, Barrington, N. J., USA). The spectrometer was a USB2000+model (Ocean Optics, Dunedin, Fla., USA). In other examples, thefluorescence excitation wavelength may be in the range of about 350 nmto about 600 nm, for example 500 nm to about 600 nm or 380 nm to about420 nm, although other ranges may also be used, including wavelengthsgreater than 600 nm and wavelengths less than 350 nm. The fluorescenceexcitation wavelength may be selected based on known characteristics ofthe excitation target, which may be, for example, a tissue target or afluorophore. For example, when the excitation target is a tissue havinga significant hemoglobin content, the excitation wavelength may beselected to be no greater than about 600 nm since hemoglobin has greaterabsorption at wavelengths less than 600 nm; for other excitation tissueor non-tissue targets which absorb wavelengths in different ranges,other excitation wavelength ranges may be used.

A data acquisition computer (e.g., a desktop computer or a laptopcomputer) may be used to control the LED signals and spectrometeracquisition. The computer may include software for carrying out dataacquisition using the system. In other examples, the system may itselfbe configured to execute such software, without communicating with anexternal processing device. In this example, the program acquired thefollowing sequence of measurements:

1. White light reflectance spectrum @ r=260 μm2. White light reflectance spectrum @ r=520 μm3. Fluorescence spectrum (405 nm excitation) @ r=260 μm4. Background signal (no light through probe)

In this example, a measurement sequence takes ˜0.5 seconds. Thesemeasurements may be used in a model for quantifying optical properties,as discussed below. In this example, the white light reflectance andfluorescence spectra obtained at r=260 μm were used for the quantitativefluorescence and spectral fitting calculations (e.g., Eqs. (5) and (7)described below). The white light reflectance spectrum obtained at r=260and 520 μm was used for the extraction of optical properties using thespectrally-constrained diffuse reflectance method (e.g., Eqs. (9)-(11)described below).

In this example, the reflectance measurements were calibrated accordingto phantoms of known optical properties such that the reflectance is inabsolute units of cm². The fluorescence measurements were calibratedaccording to a Intralipid (Fresenius Kabi: Uppsala, Sweden) and addedabsorber liquid phantom with known μ_(a,x), μ_(s,x)′ and fluorophoreconcentration.

In some examples, a phantom (e.g., a solid, sterilizable phantom) may beused as a pre-surgical calibration tool for an example of the disclosedprobe. For example, the phantom may fluoresce in the spectral range ofinterest, and may also provide background optical scattering and/orabsorption for reflectance calibration. The optical properties of thephantom may be measured using the probe (e.g., using simple lightcontact with the surface of the phantom) after absolute calibration,such as using the liquid phantom as described above. The solid phantommay provide a relative standard for fluorescence and reflectance. Sincesolid phantoms may be relatively stable, long-lasting and sterilizable(e.g., including quantum dots as fluorescent particles and/or titaniumdioxide particles for background scattering), solid phantoms may besuitable for calibration immediately prior to surgery, which may not bepossible for liquid phantoms. Determination of the quantitativerelationship between probe signals measured from the solid phantom andthe liquid phantom may allow the fluorescence and/or reflectancemeasurements of the probe to be calibrated ahead of a surgicalprocedure.

Example Model

An example model for modeling of fluorescence and reflectance detectionas mediated by the fiberoptic geometry described above is now discussed.Although certain equations and theories are described below, the presentdisclosure is not intended to be limited to these specific theories orassumptions.

Much of the research concerning the extraction of fluorophoreconcentration involves excitation wavelength(s) where the tissueattenuation is low. The challenge here is to decouple the quantitativefluorescence from the optical properties of tissue given high opticalattenuation at the excitation (relative to the emission band), sincemany fluorophores have their absorption peaks in theultraviolet-to-green spectral region, where tissue absorption is high.This example model may provide a simple, closed-form, analytical modelto extract the quantitative fluorescence spectrum with excitationwavelengths in regions of high absorption relative to the emission band.In order to extract the quantitative fluorescence, the tissue opticalproperties must be known at the excitation wavelength, which can beestimated using the spectrally-constrained diffuse reflectancetechnique. Fluorophore concentrations can then be extracted from thequantitative fluorescence spectrum through spectral decomposition usinga priori fluorescence emission basis spectra. The basis spectra areessentially the shapes of the component fluorescence spectra. Thefluorescence model may be implemented in the device and system describedabove. The example model may be useful for investigations intoaminolevulinic acid (ALA)-induced protoporphyrin IX (PpIX) tumorcontrast for guided resection surgery of brain tumors, for example;hence, PpIX is used as the target fluorophore in this example.

FIG. 7 shows a schematic of example fiberoptic-mediated fluorescencedetection. Excitation light (usually a narrow wavelength band, e.g., <50nm wide) enters the tissue through the source fiber and undergoestransport scattering and optical absorption according to the excitationoptical properties, μ_(s,x)′ and μ_(a,x). If and when excitation photonsare absorbed by fluorophores in the tissue (in this example, it isunderstood or assumed to be uniformly distributed throughout the tissueat a concentration [c]) some of the photons are re-emitted asfluorescence photons according to the fluorophore's quantum yield. Theemission photons are isotropically radiated and are transported throughthe tissue according to the emission optical properties, μ_(s,m)′ andμ_(a,m). Fluorescence emission is collected by an optical fiber at adistance r away.

The following fluorescence model is based on (but not limited to) theassumption that the optical absorption at the excitation wavelength,λ_(x), is high relative to that at the emission wavelength, λ_(m). Thisis generally true in tissue if the excitation wavelength is in theUV-blue-green end of the visible spectrum (˜350-575 nm) and the emissionwavelength is >600 nm. As a result, the fluence rate distribution at theexcitation wavelength is extremely close to the fiberoptic source;therefore, most fluorophore absorption events occur close to the sourcefiber. The migration paths of the fluorescence photons at λ_(m) can thenbe approximated as the migration paths of the reflectance photons atλ_(m) emitted and collected using the same fiberoptic geometry. Itfollows from this that the measured fluorescence, F_(x,m), has a linearrelationship with the diffuse reflectance at the emission wavelength,R_(m), with both fluorescence and reflectance measured using the samegeometry:

F _(x,m) =SR _(m)  (1)

, where the term, S, denotes the fraction of photons that are re-emittedas fluorescing photons from the total number of excitation photonslaunched into the tissue.

The term S can be modeled as the fraction of the total excitationphotons that are retained within the tissue at steady-state, S₁,multiplied by the fraction of the total absorbed photons that arere-emitted as fluorescence photons, S₂. At steady-state, the number ofexcitation photons retained within the tissue is equal to the photonsthat are not diffusely reflected out of the tissue. The fraction ofexcitation photons that are diffusely reflected is the total diffusereflectance, R_(t,x), which depends on the internal reflectionparameter, κ=(1+r_(id))/(1−r_(id)) (due to index mismatch between tissueand the external medium), and the reduced albedo at λ_(x),a_(x)′=μ_(s,x)′/(μ_(a,x)+μ_(s,x)′), which is given by diffusion theory(Flock et al., 1989):

$\begin{matrix}{R_{t,x} = {\frac{a_{x}^{\prime}}{1 + {2{\kappa \left( {1 - a_{x}^{\prime}} \right)}} + {\left( {1 + \frac{2\kappa}{3}} \right)\sqrt{3\left( {1 - a_{x}^{\prime}} \right)}}}.}} & (2)\end{matrix}$

An empirical formulation of r_(id) for index-mismatched boundaries haswidely been used, where r_(id)=−1.44n_(rel) ⁻²+0.71n_(rel)⁻¹+0.67+0.0636n_(rel), and n_(rel)=n_(tissue)/n_(external) (Groenhuis etal. 1983). For matching internal and external refractive indices, κ=1.In this example, matched indices were assumed. The blackened (with ink)epoxy surrounding the fibers in the probe acts as the external medium,and the ink-epoxy is assumed to be approximately index-matched totissue. S₁ is the fraction of photons that are not diffusely reflectedout of the tissue, so S₁=(1−R_(t,x)).

The quantitative fluorescence, f_(x,m), is defined here as the productof the wavelength-dependent fluorescence quantum yield, Q_(x,m), and thefluorescence absorption coefficient at the excitation wavelength,μ_(af,x), and is therefore an intrinsic property of the tissue, ratherthan a function of the collection geometry. The fraction of totalabsorbed photons that undergo fluorescence conversion, S₂, is simply thequantitative fluorescence divided by the total absorption:

$\begin{matrix}{S_{2} = \frac{Q_{x,m}\mu_{{af},x}}{\mu_{a,x}}} & (3)\end{matrix}$

The measured (uncorrected) fluorescence can now be expressed as:

$\begin{matrix}{F_{x,m} = {\left( {1 - R_{t,x}} \right)\left( \frac{Q_{x,m}\mu_{{af},x}}{\mu_{a,x}} \right)R_{m}}} & (4)\end{matrix}$

If the fluorophore absorption contribution is negligible compared to thetissue absorption, i.e. μ_(af,x)<<μ_(a,x), then μ_(a,x) can beapproximated to be the same as the background tissue absorption alone. Aclosed form equation for the quantitative fluorescence is:

$\begin{matrix}{f_{x,m} = {{Q_{x,m}\mu_{{af},x}} = {\left( \frac{\mu_{a,x}}{1 - R_{t,x}} \right)\left( \frac{F_{x,m}}{R_{m}} \right)}}} & (5)\end{matrix}$

Clearly, if μ_(a,x) goes to zero, the corrected, quantitativefluorescence, f_(x,m), should not go to zero. Recall the underlyingassumption that is high—Eq. (5) would be invalid at low excitationabsorption. This negates the possible scenario of μ_(a,x)=0. Note thatthe quantitative fluorescence spectrum has absolute units of nm⁻¹cm⁻¹.

Modifications to this fluorescence model are possible. For example, theabove model may be modified in order to accommodate proper operation forinterstitial measurements in addition to tissue surface measurements.This may be accomplished, for example, by forming a model of the totaldiffuse reflectance using an interstitial geometry. In another example,the model may be modified to accommodate an angled or tapered tipgeometry, such as where an angled tip is used in the probe to helpimprove the ability of the probe to push through tissue for interstitialmeasurements. As well, the S₁ factor may be computed using another meansother than diffusion theory, such as the Monte Carlo technique. Inaddition, if the fluorescence and reflectance do not perfectly scalelinearly with each other (as in Eq. (1)), a correction factor S₃ may beincluded to compensate for cases where the assumption that thefluorescence photon migration paths are similar to the reflectancephoton migration paths does not hold. S₃ may be derived via diffusiontheory, Monte Carlo or empirical techniques.

Eq. (5) yields an emission spectrum, f (μ_(m)), that can be used toquantify fluorophore concentration, c, given an a priori fluorescencebasis spectrum, b(λ), equivalent to one concentration unit [μg/mL]. Therelation is:

f=bc  (6)

, where f and b are f(λ) and b(λ) in column vector form. Taking thepseudo-inverse gives c:

c=(b ^(T) b)⁻¹ b ^(T) f  (7)

Generalizing to N fluorophores with distinct spectra, a basis matrix,B=[b₁ b₂ . . . b_(N)], can be built with the individual fluorophorebasis spectra as its columns, with a corresponding fluorophoreconcentration vector, c=[c₁ c₂ . . . c_(N)]^(T). Solving for c:

c=(B ^(T) B)⁻¹ B ^(T) f  (8)

Some assumptions in this example fluorescence model include:

1. Reflectance photons and fluorescence photons traverse similar pathlengths given the same fiberoptic distance, given that μ_(a,x)>>μ_(a,m),which is generally true if the excitation wavelength is in the highabsorption band of hemoglobin (UV-blue-green) and the emissionwavelength is in the red-to-near infrared (NIR).

2. μ_(a,x)>>μ_(af,x). In many cases, the fluorophore contribution toμ_(a,x) may be small compared to the high absorption of hemoglobin inthe range of about 350-600 nm, but this should be considered based onthe expected maximum concentration of the fluorophore of interest.

The fluorescence model of Eq. (5) requires the excitation tissue opticalproperties, μ_(a,x) and μ_(s,x)′. A method that we have established tomeasure the optical properties employs a fiberoptic source-collectorpair to measure the steady-state diffuse reflectance spectrum, as shownin FIG. 8. The following describes this technique, which may be referredto as spectrally-constrained diffuse reflectance.

FIG. 8 shows a schematic of example fiberoptic-mediated reflectancedetection. A broad-band excitation is directed into the tissue via asource optical fiber. The broad-band excitation is generally a whitelight with potentially some content in the near infrared to infraredspectral region, for example in the order of 450-850 nm wide. The lightundergoes transport scattering and absorption through the tissue that iswavelength dependent. Here, the optical properties are denoted asμ_(s,m)′ and μ_(a,m), where the emission wavelength m represents any onewavelength in the excitation broad-band. The wavelength-dependentreflectance spectrum is measured by a collector optical fiber at adistance r from the source fiber. Although there may be fluorescenceevents here, they are considered to be negligible compared with thesignal strength from the reflectance photons.

The source fiber delivers broadband white light in the spectral range ofinterest and the diffuse reflectance spectrum is detected by thecollector fiber located at a radial distance, r, from the source, andmeasured using a spectrometer. Since there is only one reflectancemeasurement per wavelength, solving for μ_(a)(λ) and μ_(x)′(λ) reliesupon spectral constraint, that is, using a priori knowledge of theshapes of the absorption and scattering coefficient spectra in theforward model. The concept here is to determine μ_(a)(λ) and μ_(s)′(λ)over a spectral range (e.g., 450-850 nm) to provide a good model fit,then extract μ_(a)(λ) and μ_(s)′(λ) by extrapolating to the excitationwavelength, which in this example λ_(x)=405 nm. Using this approach tofind the tissue optical properties requires caution that the absorptioncontributions of the fluorophores do not significantly distort thereflectance signal, and thereby the model-based curve-fitting describedbelow.

The absorption spectrum can be modeled as a linear combination of theseparate chromophore contributions. Here, it is expressed using totalhemoglobin concentration [g/L] and an oxygen saturation term:

μ_(a)(λ)=c _(Hb) [StO₂μ_(a) ^(oxyHb)(λ)+(1−StO₂)μ_(a)^(deoxyHb)(λ)]  (9)

, where μ_(a) ^(oxyHb)(λ) and μ_(a) ^(deoxyHb)(λ) are thewavelength-dependent absorption coefficients of oxygenated hemoglobin,and deoxygenated hemoglobin, respectively, for a concentration of 1 g/L.c_(Hb) is the total hemoglobin concentration and StO₂ is the oxygenationfraction. Here, water is considered negligible in the range 450-850 nm.

The reduced scattering coefficient spectrum from bulk tissue has beenshown to fit well to a simple wavelength-dependent power law, given by

μ_(s)′(λ)=Aλ ^(−b)  (10)

where A and b are constants.

The a priori knowledge of the chromophore and scatterer spectra are thencombined in a forward model of the diffuse reflectance and aLevenberg-Marquardt algorithm is then applied to extract the freeparameters. A simple approach to develop a forward model is to use thediffusion theory equation for spatially-resolved, steady-state diffusereflectance, R. Here, the radial distance, r, is fixed and the opticalproperties μ_(a)(λ) and μ_(s)′(λ) vary according to wavelength:

$\begin{matrix}{{{R(\lambda)} = {\frac{a^{\prime}}{4\pi}\begin{bmatrix}{{{z_{0}\left( {\mu_{eff} + \frac{1}{\rho_{1}}} \right)}\frac{^{{- \mu_{eff}}\rho_{1}}}{\rho_{1}^{2}}} + \left( {z_{0} + {2z_{b}}} \right)} \\{\left( {\mu_{eff} + \frac{1}{\rho_{2}}} \right)\frac{^{{- \mu_{eff}}\rho_{2}}}{\rho_{2}^{2}}}\end{bmatrix}}},} & (11)\end{matrix}$

where z₀=1/μ_(s)′, μ_(eff)(λ)=√{square root over(3μ_(a)(λ)μ_(s)′(λ))}{square root over (3μ_(a)(λ)μ_(s)′(λ))}, μ_(a)(λ)and μ_(s)′(λ) are given by Eqs. (9) and (10), ρ₁ ²=z₀ ²+r² and ρ₂²=(z₀+2z_(b))²+r². The parameters z₀, r₁, r₂, z_(b) and μ_(eff) are allwavelength-dependent. The z_(b) factor depends on μ_(a), μ_(s)′ and theinternal reflection parameter κ. The extrapolated boundary distance isgiven by z_(b)=2 κD, where D is the diffusion constant given byD=(3μ_(s)′)⁻¹. This version of the diffusion constant was selected forreasons given in previous studies on the measurement of tissue opticalproperties. For matching internal and external refractive indices, κ=1,which was assumed in this example, although this is not a requirement ingeneral.

The free parameters are, therefore, the total hemoglobin concentration,oxygen saturation and scattering parameters. This is not quite as simpleas applying the inverse algorithm to any r; for each r, there is a rangeof validity that is constrained by the peak of the reflectance versusμ_(s)′; curve, and the diffusion model breakdown at low μ_(s)′. By usingreflectances measured at several r, the ranges of validity overlap, thusincreasing the total dynamic range. In this example, r=260 and 520 μmwere selected. It has been found that for these values of r, thevalidity range for the spectral constraint technique wasμ_(s)′=10.1-47.4 cm⁻¹. FIG. 11 shows the lower and upper bounds ofμ_(s)′ (for μ_(a)<10 cm⁻¹) where each fiberoptic distance is valid(e.g., for r=260, 520 and 780 μm, the 780 μm distance shown todemonstrate how a longer distance does expand the overall dynamicrange).

The r=260 and 520 μm source-collector distances were used because inthis example the brain is the target site of interest and the brainoptical properties have been measured as within this range of validityin previous laboratory experiments on murine tissues.

Variations to the described reflectance model may be possible. Forexample, as in the case with the fluorescence model, the abovereflectance model may be modified in order to accommodate properoperation for interstitial measurements in addition to tissue surfacemeasurements. This may be accomplished, for example, by forming a modelof the diffuse reflectance using an interstitial geometry. In anotherexample, the model may be modified to accommodate the geometry of anangled or tapered probe tip, for example where the probe has an angledtip to help the probe to push through tissue in order to takeinterstitial measurements. Further, as with the quantitativefluorescence model, the light-tissue interaction may be modeled withdiffusion theory, Monte Carlo techniques or empirical techniques.

A technique that was found to relatively accurately determine theoptical properties is to calculate μ_(a)(λ) and μ_(s)′(λ) for eachfiberoptic distance (in this example, this is r=260 and 520 μm) and todetermine which r distance to use for the calculation by checking if theμ_(s)′(λ) value falls within the upper and lower bounds of validity (forexample, as shown in FIG. 11).

Example Studies Phantom Studies

Phantom experiments were carried out to validate the examplefluorescence model described above. Intralipid fluid (Fresenius Kabi,Uppsala, Sweden) was used to provide background scattering. Yellow foodcoloring (McCormick Canada, London, ON, Canada) was used to vary theabsorption coefficients. Protoporphyrin IX extract (Sigma-Aldrich) wasused as the target fluorophore. A set of nine phantoms were mixed,giving the optical properties shown in FIG. 12.

PpIX was mixed in six concentrations (5, 2.5, 1.25, 0.625, 0.3125,0.15625 μg/mL) for each set of nine phantoms, for a total of 54phantoms. Probe measurements were taken in each of the 54 phantoms, andEq. (5) applied to the data to extract the quantitative fluorescencespectra and the PpIX concentration. As well, images of the liquidphantom surfaces in blackened cuvettes were taken using a fluorescencestereomicroscope (MZ FLIII: Leica, Wetzlar, Germany) to determine thefluorescence image intensity variation at [PpIX]=5 μg/mL.

This set of experiments was used to validate the example fluorescencemodel in Eq. (5) and (7), with a priori knowledge of the excitationoptical properties. The measured fluorescence spectra, F_(x,m)(λ_(m))for the set of nine phantoms A-I, all with a PpIX concentration of 5μg/mL, is shown in FIG. 13 a. The raw measurements of FIG. 13 a have notbeen corrected for optical properties variations. Applying Eq. (5) tothe data produces the quantitative fluorescence spectra, f (λ_(m)),shown in FIG. 13 b, with correction for the effects of opticalproperties. The relative standard deviation (normalized to the mean) atthe 635 nm peak is 53.1% for the measured fluorescence and 10.1% for thequantitative fluorescence estimate.

The quantitative fluorescence model was applied to the entire data setof 54 phantoms, with the results plotted against PpIX concentration.FIG. 14 a shows the measured, raw fluorescence intensities at 635 nm,compared to the estimated [PpIX] concentration shown in FIG. 14 b,calculated from the raw data using Eqs. (5) and (7). The dashed line ina) represents the best straight line fit through the origin to the data;the dashed line in b) is the unity line. R² calculations were performedon the linear form of the data plot, not the log-log relation. Themeasured probe signal F_(x,m)(λ_(m)=635 nm) was curve-fitted to a linearmodel with [PpIX] on the x-axis in FIG. 14 a. The [PpIX] probe estimatewas compared to the known [PpIX] in FIG. 14 b. The root-mean-square(RMS) variation from the mean, and maximum deviation from the mean werecalculated from the data at 5 μg/mL (FIG. 13). The RMS error and maximumerror were normalized by the mean. Using the quantitative fluorescencemodel and spectral fitting, the estimated [PpIX] concentration has anRMS deviation of 10.1% and a maximum deviation of 14.2%, representing asignificant decrease in error from the uncorrected fluorescencemeasurement (RMS deviation 52.3% and maximum deviation 94.9%).

Fluorescence microscope images of the phantoms were taken in order toget a visual conception of the fluorescence intensity variation due tochanges in optical properties. Phantom surface images are shown in FIG.15 for [PpIX]=5 μg/mL, the intensity differences between phantoms may beeasily distinguishable by eye. The most significant intensity differencein this set of images is between phantom C (highest μ_(s,x)′, lowestμ_(a,x)) and G (lowest μ_(s,x)′, highest μ_(a,x)), where thefluorescence intensity of C is 4.0 times that of G.

Mouse Model

In another example study, a mouse tumor model was used to validate theexample probe's accuracy in measuring photosensitizer concentrations invarious tissue types, with PpIX as the target marker. The probe estimateof [PpIX] was compared with measurements of diluted, solubilized tissuein a cuvette-based fluorometer, based on a published protocol (Lilge etal., 1997)

Tumor Induction:

Five male mice (20 grams) were anesthetized with 2% isoflurane andplaced on a warming blanket. The skin at the injection site was swabbedwith 70% ethanol, and 10⁶ B16 melanoma cells in 20 μL of phosphatebuffered saline were injected subcutaneously into the left flank. Tumorswere allowed to grow 4-6 mm over 7 days.

PpIX Measurement in Various Organ Tissues:

After tumors had grown to size, each mouse was injected via tail veinwith 100 mg/kg ALA at 0.5, 1, 2, 3 and 4 hours prior to sacrifice. Thedifferent time points were selected to ensure a large range of [PpIX] ineach tissue. The mice were sacrificed by cervical dislocation whileunder isoflurane anesthesia. The tissue types of interest (brain, heart,kidney, liver, muscle, skin and tumor) were rapidly excised undersubdued lighting conditions and three probe measurements taken pertissue sample. The samples were weighed, placed into cryotubes and thensnap frozen in liquid nitrogen. The samples were stored at −70° C. in alight-tight container until ready for the tissue solubilisationprocedure.

Tissue Solubilisation Protocol:

A tissue solubilisation protocol was used to measure the absolutefluorophore concentration (Lilge et al, 1997). Each tissue sample wascombined with 2 mL of Solvable and placed in an undulating water bath at50° C. for 1 hour. The tissue/Solvable solution was homogenized with aTissue Tearor tool (Biospec Products, Bartlesville, Okla., USA) in theoriginal vial. 200 μL of the tissue homogenate was combined with 3 mL ofdistilled water and 1 mL of Solvable. This solution was incubated in thewater bath at 50° C. for 1 h. The optical density was measured anddiluted down to <0.1 if necessary. The resulting solution wastransferred to a quartz cuvette. The cuvette was analysed viafluorometer (Fluorolog: Jobin Yvon, Edison, N.J., USA), using anexcitation wavelength of 401 nm. A look-up curve was constructed bymeasuring known concentrations of PpIX in 75/25 distilled water/Solvablesolution, with the detector nonlinearity taken into account for the[PpIX] calculations.

FIG. 16 a shows the measured (i.e., uncorrected) fluorescence signalexemplified by the PpIX peak at 635 nm plotted against the [PpIX]measurement from the tissue solubilisation technique for 34 tissuesamples. The y-axis has been re-scaled mid-figure to better visualizethe higher-value data. The data show significant scatter, with littlecorrelation between the x- and y-axes. Applying the example correctionalgorithms described above to the data, the resulting [PpIX] estimatefrom the fiberoptic probe has improved correlation with the [PpIX]measurement from the tissue solubilisation technique (FIG. 16 b),demonstrating that the example fiberoptic probe an associated model andmethod described above may be useful for quantifying fluorophore contentin tissue. In FIG. 16 a and FIG. 16 b, y-axis error bars are standarddeviation bars from three measurements on each tissue sample; x-axiserror bars were calculated from the root-mean-square (RMS) errorpercentage from accuracy statistics of the solubilisation protocol(Lilge et al., 1997), multiplied by 0.5 of the maximum [PpIX] measuredvia tissue solubilisation in this mouse tissue experiment.

Example Patient Study

Another example study is now described. In this example study, 14patients with a variety of intracranial pathologies (including low- andhigh-grade gliomas, meningioma, and intracranial lung metastases, asindicated in FIG. 17) were administered 20-mg/kg of ALA 3 hours prior toinduction of anesthesia. The neurosurgeon performed a conventionalstandard craniotomy procedure with image-guidance using a surgicalmicroscope modified for PpIX fluorescence. At the surgical bed, thesurgeon acquired multiple sets of measurements using an example probe,with corresponding tissue specimens collected and processed forhistopathological analysis. In each case, the surgeon acquired probemeasurements of normal brain tissue as controls. In addition, thesurgeon scored the visible fluorescence as viewed through thefluorescence-capable surgical microscope (scores from 0-4). Allspecimens were assessed as either normal or abnormal, such that presenceof tumor cells was assessed as abnormal. The total numbers of controland biopsy sites are listed in FIG. 17.

Statistical significance tests, linear discriminant analysis andreceiver-operator characteristic analysis were performed on this in vivodata set acquired by the example quantitative fluorescence probe.

Statistical Significance Tests:

Since the data in this example were expected to be non-parametric, aWilcoxon rank-sum test was selected in order to determine howstatistically-significant were the several optical parameters derivedfrom the in vivo probe data at distinguishing between normal and tumortissues. PpIX concentration was one of the parameters to be tested. Inaddition, the following optical parameters were tested for statisticalsignificance in differentiating normal from tumor tissue: theautofluorescence (AF) at 600, 635, 650 and 700 nm, diffuse reflectance(for both r=260 and 520 μm fiber distance) at 575 and 600 nm, oxygensaturation (StO₂), total hemoglobin concentration, f_(Hb), and μ_(a) andμ_(s)′ at 575 and 600 nm.

Linear Discriminant Analysis:

Statistically-significant optical parameters that disprove the nullhypothesis according to Wilcoxon rank-sum tests were evaluated as totheir physiological and photochemical relevance to brain cancer. Theselected parameters were used in a linear discriminant analysis (i.e.Fisher's linear discriminant) in order to find the vector in thisfeature space such that the normal and tumor classes were separated to amaximal extent (at least, in a linear fashion).

Receiver-Operator Characteristic Analysis:

The receiver-operator characteristic (ROC) curves were generated usingthe PpIX concentrations as the parameter comparing normal tissue tothese tumor populations: all tumors, all gliomas, low-grade gliomas(LGGs), high-grade gliomas (HGGs), meningiomas and metastases. Optimalsensitivity and specificity values were extracted to determineperformance of PpIX concentrations as a tumor-specific marker. Theperformance of PpIX concentrations as a tumor-specific marker wascompared with a metric for the uncorrected, raw fluorescence spectrum(i.e., not corrected for optical properties), and the neurosurgeon'sscoring of the visible fluorescence through the surgical microscope(scores are from 0 to 4). The uncorrected fluorescence metric was themagnitude of the PpIX fluorescence peak from the raw fluorescencespectrum, i.e. F_(x,m) at 635 nm (see Eqs. 1 and 4). In this example,the three qF probe measurements at each site were used separately tocalculate the ROC curve (rather than averaging the triplicatemeasurements at each site).

As well, ROC analysis was performed for the above pathologies usinglinear discriminant analysis. In other words, multiple variables thatcan be quantified from the probe data (e.g., PpIX concentration, oxygensaturation, hemoglobin concentration, etc.) were used to attempt toseparate the normal and tumor classes to the maximum extent. Since the“training” data set used to train the LDA should not be the same as the“validation” data set used to evaluate the LDA using ROC analysis, across-validation algorithm was set up to assess the performancestatistics. The cross-validation scheme that was used was repeatedrandom sub-sampling validation. Essentially, half of the data set israndomly sampled and assigned as the training data set to train the LDA.The remaining half is used for validation using ROC analysis. Theprocess was repeated several times and the performance statistics wereaveraged. In this example, the random sampling process was run 50 times.

Results

In Vivo Probe Measurements:

The data fits to the reflectance and fluorescence measurements from thehandheld probe during resection surgery were generally good across alltissues. FIG. 18 displays data from an example patient with a high gradeglioma (HGG) and an example meningioma patient. Panels (a) and (b)display measurement data in normal tissue (in this case, normal brainparenchyma), while panels (c) and (d) show measurement data from tumortissue (in this case, a high-grade glioma). Panels (a) and (c) show invivo tissue optical properties spectra, while panels (b) and (d) showreflectance data with model fit. The reflectance model fits very closelyto the reflectance measurements. FIG. 19 shows quantitative in vivofluorescence data from an example meningioma patient, as measured usingan example handheld fluorescence probe. Panels (a) and (b) show datafrom the meningioma, while panels (c) and (d) show data from normaldura. Panels (a) and (c) show examples of the quantitative fluorescencemeasurement and model fit, while panels (b) and (d) show examples of thespectrally unmixed signals. The tumor data show a strong PpIX signal;however, the normal tissue data show AF in the same range as the PpIXsignal. These demonstrate the usefulness of accurate AF modeling: inthis neurosurgical trial, the AF was modeled as a linear combination ofthe spectral shapes of flavin adenine dinucleotide, nicotinamide adeninedinucleotide and lipofuscin.

FIG. 20 exemplifies the additional sensitivity to fluorescence that maybe afforded by the disclosed probe as compared to the fluorescencemicroscope during resection surgery. In FIG. 20, images and measurementswere obtained from an example low-grade glioma patient during tumorresection surgery. Panels (a) and (b) display fluorescence images fromtwo sites in the same patient that were both histologically confirmed astumor. Panels (c) and (d) quantitative fluorescence spectra, obtainedusing an example fluorescence probe, corresponding to the sites ofpanels (a) and (b), respectively. The probe measurement sites areindicated by white arrows, and the probe shaft is visible at the pointof contact. The first site (panels (a) and (c)) shows visiblefluorescence (in this case obtained using an operating microscope), withthe surgeon scoring that site as a ‘2’. This was confirmed by the probemeasurement with [PpIX]=12.9 μg/mL. However, the second site (panels (b)and (d)), measured after the visible tumor from the first site wasremoved, has no visible fluorescence (surgeon's score of ‘0’) yet thereis a measureable [PpIX] of 0.36 μg/mL Both sites were histologicallyconfirmed as tumor tissue.

FIG. 21 is a table displaying example PpIX concentrations measured invivo by an example probe in normal and tumor tissues for the variouspathologies. The table of FIG. 21 shows example comparisons of in vivoPpIX concentration levels found in normal and tumor tissues for each ofthe pathologies in the example study. The tumor-to-normal (T/N) ratiosfor all pathologies, in this example, are of the same order of magnitudeand average out to T/N=200. Standard deviations are indicated by thesymbol “±”.

In this example, fifteen optical parameters were tested as to theirstatistical significance in differentiating between normal and tumortissue in vivo. The table of FIG. 22 shows the list of examplediagnostic variables that were tested for significance indifferentiating between normal and tumor tissues. In this table, thevalue h denotes if the null hypothesis has been disproved, with ‘1’indicating the null hypothesis is not true and ‘0’ indicating it istrue. The range of significance levels used to reject the nullhypothesis was p<0.05. This analysis was performed for the glioma dataset and the all-tumors data set. Partly based on this, [PpIX], AF at 600nm, the reflectance at both fiber separations at 600 nm, StO₂ and f_(Hb)were used for the multi-variable linear discriminant analysis.

There may also be physiological and photochemical reasons for theseselections for multi-variable linear discriminant analysis. [PpIX] maybe the chief tumor biomarker. Autofluorescence (AF) may betumor-specific, and the autofluorescence at 600 nm (in this example, theshortest wavelength in the fluorescence data collection range) may havethe strongest AF signal, since AF may peak in the green region of thespectrum, which is near 600 nm. FIG. 23 shows examples of cluster plots(for the example all-tumors data set and the example HGG data set) withAF on the y-axis and [PpIX] on the x-axis, illustrating (at leastqualitatively, visually) that autofluorescence may be useful inseparating the normal and tumor data clusters. In FIG. 23, (a) is a plotshowing example data for all tumors studied and (b) is a plot showingexample data for high-grade gliomas. Reflectance values at 600 nm may bedominated by scattering, which in turn may be affected by cell organellesize and morphology. Reflectance measurements at the two fiberseparations may contain encoded information on not only the reducedscattering coefficient, but also the scattering phase function. Finally,f_(Hb) and StO₂ may be diagnostically useful in distinguishing betweenglioma and normal tissues.

ROC Analysis of the In Vivo Probe Data

FIG. 24 displays the example ROC curves for (a) the in vivo all-tumorsdata set and (b) the in vivo HGG data set. In FIG. 24, the examplediagnostic variables considered were: the surgeon's visible fluorescencescore as determined through the surgical microscope; the PpIX peak(e.g., 635 nm) fluorescence magnitude from the raw, uncorrectedfluorescence spectrum; PpIX concentration; and a metric derived fromlinear discriminant analysis. In this example, the raw uncorrectedfluorescence measurement at the 635 nm PpIX peak performed the worst ofthe variables studied. The surgeon's subjective fluorescence scoring wasbetter than the raw fluorescence metric. Note that the surgeon in thisexample study was relatively experienced with ALA-PpIX FGR, so the goodperformance of the subjective fluorescence scoring result may beunrepresentative. However, the quantitative [PpIX] metric performedbetter than the surgeon's scoring, with the LDA model improving thetumor detection accuracy.

FIGS. 25-29 displays example ROC in vivo data, including the ROC areaunder the curve (a.u.c.) data, sensitivity and specificity values for:the all-tumors (FIG. 25), HGG (FIG. 26), LGG (FIG. 27), intracraniallung metastasis tumors (FIG. 28) and meningioma (FIG. 29) data sets. Inthis example study, for all tissue types with the exception ofmetastatic tumors, the general trend holds that the order of increasingperformance is: raw fluorescence at 635 nm, surgeon's score, [PpIX] andthen LDA. For the lung metastatic tumor data, the ROC area-under-curveis slightly higher for [PpIX] than the LDA classifier, though both thesehave better performance than the surgeon's score and raw fluorescencemetric.

Conclusion:

In this example study an intraoperative fiber-optics probe was used toestimate PpIX concentrations in vivo during clinical intra-cranialresection procedures. Spectrally-dependent endogenous optical properties(e.g., absorption, scattering) were computed for each light collectionpoint and used as prior information in an algorithm designed to estimatePpIX concentrations from measured fluorescence spectra.

There may be several clinical rationales for using an example of thedescribed probe. For example, it may be used to overcome limitations ofconventional fluorescence imaging techniques and instruments used (suchas the lack of quantification of the fluorescence signal in a typicalimaging system), for example, for surgical guidance. Thus, an example ofthe probe may be used to detect lower concentrations of fluorophore intissue and provide better discrimination from the autofluorescencetissue background. An example of the probe may also provide quantitativeand absolute measurements of the fluorophore concentration in thetissue. An example of the probe may also provide relatively highlylocalised measurements, which may be at the tissue surface or at depth.For example, depth measurements may be acquired by positioning the probetip interstitially through the tissue, to allow interstitialmeasurements to be taken.

The example study described above shows evidence that probe measurementsmay confer greater sensitivity than the surgical microscope in detectinga significant amount of PpIX fluorescence in abnormal tissue compared tonormal. In instances of no visible fluorescence as determined throughthe surgical microscope, probe estimates of PpIX concentrations in thisstudy showed approximately at least five times more PpIX in abnormaltissue compared to normal tissue. It may also be useful to corroboratethe diagnostic capabilities of using probe estimates of PpIXconcentration as an intraoperative diagnostic tool for delineating tumormargins in ALA-PpIX FGR. This example study illustrates thatquantitative measurements using the example probe may be sensitiveenough to detect a significant difference between abnormal tissue (e.g.,tissue with the presence of tumor cells and/or reactive changes) andnormal tissue. It may be useful to note that these reactive changes maybe present in the peritumoral regions and as such may providepreliminary data regarding the probe's ability to detect PpIXdifferences at the farthest extent of tumor margins. To summarize, theresults of this example study may provide data to support usingspectroscopically estimated PpIX concentrations for tumor margindelineation and in vivo diagnosis.

The diagnostic accuracy of the example probe may be further improved byconsidering diagnostic variables other than PpIX concentration. Forexample, other metrics such as reflectance, oxygen saturation,hemoglobin concentration and/or autofluorescence may be included in thediagnostic determination. FIGS. 24-29 show examples of how a diagnosticdetermination taking multiple diagnostic variables into account may beuseful (in these example figures, linear discriminant analysis was foundto generally have a relatively good diagnostic result).

Applications

The example device and system described, and associated model and methodmay provide useful diagnostic techniques for the operating room. Forexample, the fiberoptic probe may be useful as an intraoperativediagnostic tool for delineating brain tumor margins. For example, FIG.20, panels (a) and (b) illustrate the use of the probe in a tumor site.The probe measurement sites are indicated by white arrows, and the probeshaft is visible at the point of contact.

There may be a number of biomedical applications for in situquantitative fluorescence spectroscopy using the described device,system and method. Although the above discussion focused primarily onthe use of the example fiberoptic probe to delineate glioma tumormargins during resection surgery, the probe can be used for generaloptical diagnosis or monitoring of tissue disease states or normalphysiology. Endogenous and exogenous fluorescence contrast has beenexplored to detect and diagnose diseased tissue. Since tissue opticalproperties and measurement geometry may significantly affect thefluorescence signal, it is useful for these distorting effects beremoved. The disclosed device, system and method may therefore be usefulfor improving current efforts to correctly diagnose or monitor tissueusing fluorescence.

Another potential application is the evaluation of drug biodistributionand time kinetics in patients and pre-clinical animal models. It isoften useful to know how a drug is distributed in various organs andpathological tissues for diagnostic, therapeutic or response monitoringclinical applications. Many diagnostic and therapeutic photosensitizingdrugs are fluorescent, such as protoporphyrin IX (PpIX), Photofrin andbenzoporphyrin derivative. Alternatively, the drug may happen to befluorescent despite the clinical application of the drug being unrelatedto fluorescence: for example, the chemotherapy drugs taxol,cyclophosphamide and doxorubicin are fluorescent. Alternatively, a drugmay be made fluorescent by tagging it with a fluorescent reporter, suchas binding fluorescent molecules (or molecular beacons, ornanoparticles) to chemotherapy drugs, heart medication, or othermedications. It is a common goal in pre-clinical studies to measure drugcontent in various organs to determine the safety and efficacy of thedrug. The example device and system may be useful to determine the timekinetics and biodistribution of such fluorescing drugs, such as a recentpre-clinical study done in our labs with a porphyrin dimer-based drugfor two-photon absorption photodynamic therapy of melanoma tumors.

The fiberoptic probe can also be used to monitor photodynamic therapy(PDT). There are several physiological parameters that may be used todynamically monitor PDT, such as tissue oxygenation, fluorescent drugconcentration, etc. Another form of implicit PDT monitoring may be basedon monitoring the generation of photoproducts of the therapy drug. PpIXhas known photoproducts with spectral peaks distinct from the originalfluorophore. PpIX photoproduct generation has been shown due tounintentional photodynamic therapy of brain tumors by the illuminationfrom the neurosurgical microscope. This phenomenon may also be a usefulmetric for monitoring of PDT (i.e. done intentionally). Implicit PDTmonitoring of oxygen and drug depletion may aid in reducingpatient-to-patient variability.

In some examples, the present disclosure may be useful for radiationtherapy, for example to detect target tissue for treatment and/or toassist in identifying tumor tissue for designing a radiation treatmentplan. The present disclosure may also be useful for delineating marginsin various tumor sites including, for example, the head and neck, theprostate, the breast, skin, and other sites.

This technique may be expanded to non-biological or non-living tissueapplications, for example where fluorescence quantification may be usedto test optically-turbid, fluorescent materials. Examples of suchmaterials include but are not limited to: pulp and paper, food andbeverage, paint making, plastics, lumber, food safety (e.g., detectionof food-borne bacteria or pathogens that may be fluorescent or made tobe fluorescent), and pharmaceuticals (e.g. pills). Fluorescencequantification may be used for quality control of materials or for humansafety purposes.

There may be additional functionality applied to the fiberoptic probe. Aswitch on the probe or a foot pedal may be added to trigger dataacquisitions. As well, the measurement cycle and algorithm computationschematically represented in FIG. 9 may be run continuously to providereal-time information to the operator. The example shown in FIG. 9includes measurement of: the fluorescence spectrum (at one fiberopticdistance), the reflectance spectr(a)um (at one or multiple fiberopticdistances) and the background ambient light. The diagram also outlinesthe example algorithm to compute the optical properties, quantitativefluorescence and fluorophore concentrations, to be elucidated in thedetailed description section.

Optical tracking or electromagnetically tracking sensors may be placedon the probe to track its position and orientation with reference toother imaging modalities and surgical tools. One example with regard tosurgical tumor resection is to use position tracking sensors to trackthe probe within a surgical cavity (e.g., a brain cavity) with referenceto pre-operative MRI or CT, to correlate fluorescence point measurementswith tomographic information.

Other techniques may be used to estimate the optical properties. Forexample, in the UV-blue region, hemoglobin absorption may besignificant, so a photothermal technique (such as pulsed-photothermalradiometry or photoacoustic spectroscopy) may be used to measure theexcitation absorption and scattering. Photothermal optical propertiesmeasurements have a larger dynamic range than diffuse reflectance formeasuring absorption because in the former, optical absorption adds tothe measurement signal whereas in the latter, absorption subtracts fromthe signal, which makes the maximum measureable absorption restricted bythe signal-to-background contrast.

The described device, system and methods may be used where fluorescenceemission is detected from a fluorescence marker, including, for example:protoporphyrin IX (PpIX) (including ALA-induced PpIX) and indocyaninegreen (ICG). Other suitable fluorescence markers may include, forexample: an organic fluorophore (e.g., nicotinamide adeninedinucleotide, flavin adenine dinucleotide, or collagen), ananoparticle-based agent (e.g., a quantum dot, or a nanoparticlecarrying a fluorescent agent.), fluorescein and a fluorescent molecularbeacon (e.g., based on enzymatic cleavage or antisense hybridization).The fluorescence marker may be targeted to a tissue of interest using atargeting moiety, such as an antibody or a peptide. The fluorescencemarker may alternatively be untargeted. In some examples, thefluorescence marker may be fluorescein (e.g., for marking disruptedblood-brain barrier of a brain tumor).

Although certain examples have been described, these are for the purposeof illustration only and are not intended to be limiting. Variations,combinations, and equivalents of the specific embodiment, method, andexamples herein may be possible. Features described in separate examplesmay be used in combination. Specific values and sub-ranges withindisclosed ranges are also disclosed. The present disclosure is notnecessarily bound by any theory or assumptions described by way ofexample. All references mentioned are hereby incorporated by referencein their entirety.

REFERENCES

-   1. K. R. Diamond, M. S. Patterson, T. J. Farrell. “Quantification of    fluorophore concentration in tissue-simulating media by fluorescence    measurements with a single optical fiber,” Appl. Opt. 42(13),    2436-2442 (2003).-   2. J. C. Finlay, T. C. Zhu, A. Dimofte, D. Stripp, S. B.    Malkowicz, T. M. Busch, S. M. Hahn. “Interstitial Fluorescence    Spectroscopy in the Human Prostate During Motexafin    Lutetium-Mediated Photodynamic Therapy,” Photochem. Photobiol.    82(5), 1270-1278 (2006).-   3. S. T. Flock, M. S. Patterson, B. C. Wilson, D. R. Wyman.    “Monte-Carlo modeling of light-propagation in highly scattering    tissues: 1. Model predictions and comparison with diffusion-theory,”    IEEE Trans. Biomed. Eng. 36(12), 1162-1168 (1989).-   4. F. A. J. Groenhuis, H. A. Ferwerda, J. J. ten Bosch. “Scattering    and absorption of turbid materials derived from reflection    coefficients. 1: Theory,” Appl. Opt. 22(16), 2456-2462 (1983).-   5. L. Lilge, C. O'Carroll, B. C. Wilson. “A solubilization technique    for photosensitizer quantification in ex vivo tissue samples,” J.    Photochem. Photobiol. B 39(3), 229-235 (1997).-   6. M. G. Müller, I. Georgakoudi, Q. Zhang, J. Wu, M. S. Feld.    “Intrinsic fluorescence spectroscopy in turbid media: disentangling    effects of scattering and absorption,” Appl. Opt. 40(25), 4633-4646    (2001).-   7. R. Sroka, W. Beyer, L. Gossner, T. Sassy, S. Stocker, R.    Baumgartner. “Pharmacokinetics of 5-aminolevulinic-acid-induced    porphyrins in tumour-bearing mice,” J. Photochem. Photobiol. B,    Biology 34(1), 13-19. (1996).-   8. W. Stummer, H. Stepp, G. Moller, A. Ehrhardt, M. Leonhard, H. J.    Reulen. “Technical principles for protoporphyrin-IX-fluorescence    guided microsurgical resection of malignant gliomas tissue,” Acta    Neurochir. 140(10), 995-1000 (1998).-   9. R. Weersink, M. S. Patterson, K. Diamond, S. Silver, N. Padgett.    “Noninvasive measurement of fluorophore concentration in turbid    media with a simple fluorescence/reflectance ratio technique,” Appl.    Opt. 40(34): 6389-6395 (2001).-   10. J. Wu, M. S. Feld, R. P. Rava. “Analytical model for extracting    quantitative fluorescence in turbid media,” Appl. Opt. 32, 3585-3595    (1993).

1. An optical probe for quantifying fluorescence and optical propertiesin tissue, the probe comprising: a probe body and a probe tip at adistal end of the probe body, the probe tip being configured tosubstantially contact a target surface; at least one detector at theprobe tip for detecting fluorescence emission or reflectance wavelengthsfrom the target surface; at least one fluorescence excitation source atthe probe tip for providing fluorescence excitation light to the targetsurface, each one of the at least one fluorescence excitation sourcebeing at a known distance from each one of the at least one detector; atleast two broadband sources at the probe tip for providing broadbandwavelengths to the target surface, each one of the at least twobroadband sources being at a known distance from each one of the atleast one detector; wherein the device is configured to communicatesignals from the at least one detector representing the detectedwavelengths to a processing device.
 2. The probe of claim 1 wherein atleast one of the broadband sources is at a distance from the at leastone detector that is the same as a distance between the at least onefluorescence excitation source and the at least one detector.
 3. Theprobe of claim 2 wherein there is one detector, one fluorescenceexcitation source at a distance of about 260 micrometers from thedetector, and two broadband sources at a distance of about 260micrometers and about 520 micrometers, respectively, from the detector.4. The probe of claim 1 wherein the fluorescence excitation source isconfigured to provide fluorescence excitation light in the range ofabout 350 nm to about 600 nm.
 5. The probe of claim 4 wherein thefluorescence excitation source is configured to provide fluorescenceexcitation light in the range of about 500 nm to about 600 nm.
 6. Theprobe claim 4 wherein the fluorescence excitation source is configuredto provide fluorescence excitation light in the range of about 380 nm toabout 420 nm.
 7. The probe of claim 1 wherein the detector is configuredto detect emission or reflectance wavelengths in the range of about 450to about 850 nm.
 8. The probe of claim 1 wherein the probe tip has anangled or tapered configuration.
 9. The probe of claim 1 wherein thereis a plurality of fluorescence excitation sources at the probe tip, eachof the plurality of fluorescence excitation sources providing adifferent range of fluorescence excitation wavelengths.
 10. The probe ofclaim 1 wherein the target surface is a tissue surface locatedinterstitially within the tissue.
 11. A method for quantifying opticalproperties in a turbid medium, the method comprising: providingfluorescence emission and reflectance wavelengths detected from a targetsurface, each of the detected wavelengths being associated with arespective known distance between a respective excitation source givingrise to the respective detected wavelength and a detector detecting therespective detected wavelength, the known distances being predeterminedto enable calculation of a desired range of values for the opticalproperties; and calculating the optical properties based on the detectedwavelengths and the respective known distances, and based on a model oflight interaction with the turbid medium, the model limiting a range ofcalculated values for the optical properties.
 12. The method of claim 11wherein there are at least two reflectance wavelengths having differentrespective known distances between the excitation sources and thedetector; and wherein at least one of the at least two reflectancewavelengths has a respective known distance equal to a respective knowndistance for at least one emission wavelength.
 13. The method of claim11 or claim 12 further comprising selecting the reflectance wavelengthsfor calculating the optical properties, and comparing the reflectancewavelengths with one of: a known absorption spectrum and fluorescencequantum yield of a fluorophore; and a measurement in the turbid mediumof a known fluorophore concentration.
 14. The method of any one ofclaims 11 to 13 further comprising calculating a quantitativefluorescence spectrum with the calculated optical properties used in acorrective algorithm.
 15. The method of claim 14 further comprisingcalculating fluorophore concentrations using spectral decomposition ofthe quantitative fluorescence spectrum.
 16. The method of any one ofclaims 11 to 15 wherein the detected wavelengths include detectedemission wavelengths from a fluorescence marker.
 17. (canceled) 18.(canceled)
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 27. Themethod of claim 11 wherein the turbid medium is tissue.
 28. The methodof claim 10 wherein quantifying optical properties comprises quantifyingfluorescence in a turbid medium, the method further comprising:providing quantified optical properties corresponding to the detectedwavelengths; and calculating the fluorescence based on the detectedwavelengths and the corresponding optical properties, based on knownrelationships between the detected emission wavelengths and the detectedreflectance wavelengths arising from the known distances, and based on amodel of light interaction with the turbid medium, the model limiting arange of calculated values for the optical properties.
 29. (canceled)30. The method of claim 28 further comprising calculating fluorophoreconcentrations using spectral decomposition of the calculatedfluorescence.
 31. The method of claim 28 wherein the detectedwavelengths include detected emission wavelengths from a fluorescencemarker.
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 35. (canceled) 36.(canceled)
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 42. (canceled)
 43. The method of claim 28 furthercomprising calibrating the detector using a phantom to provide areference fluorescence measurement for calculating the fluorescence. 44.(canceled)
 45. (canceled)
 46. A system for quantifying opticalproperties in tissue, the system comprising: the probe of claim 1; aplurality of light sources for providing fluorescence and broadbandwavelengths to the fluorescence excitation source and broadband sources;a spectrometer for measuring wavelengths detected by the detector; and acontroller for controlling emission and detection by the sources and thedetector; wherein the system is in communication with a processingdevice configured for calculating at least one optical property based onthe measured wavelengths.
 47. (canceled)
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 53. (canceled) 54.(canceled)